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pvdf membrane for anillin  (Bethyl)
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Bethyl pvdf membrane for anillin
Figure 1. <t>Anillin</t> immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).
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Figure 1. Anillin immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).

Journal: Oncology Reports

Article Title: Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma

doi: 10.3892/or_00001051

Figure Lengend Snippet: Figure 1. Anillin immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).

Article Snippet: After boiling the samples in reducing SDS sample buffer for 5 min, 10 μg of protein was loaded per lane and the samples were electrophoresed on 10% polyacrylamide SDS gel and transferred to a PVDF membrane for anillin (IHC-00298, Bethyl Laboratories) or lamin A/C (NCL-LAM-A/C, Leica Biosystems Newcastle Ltd.) detection.

Techniques: Immunostaining, Immunohistochemical staining, Staining, Expressing, Negative Staining, Control

Figure 2. Anillin is expressed in cytoplasmic and nuclear fractions of the RCC cell line 786-O. Cytosolic (lane 1) and nuclear (lane 2) fractions were isolated and 20 μg of protein was loaded onto the SDS-PAGE gel. Western blotting with anillin antibody staining (upper blot) mainly shows expression in the nuclear fraction, but some expression is present in the cytosolic fraction. The size of anillin protein was identical in both fractions, ~120 kDa (upper blot and data not shown). Lamin A/C expression (lower blot) is present only in the nuclear fraction, excluding contamination of the cyto- solic fraction with nuclear content. Molecular weight markers (in kDa) are shown in the right margin. Representative blots from repeated experiments are shown.

Journal: Oncology Reports

Article Title: Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma

doi: 10.3892/or_00001051

Figure Lengend Snippet: Figure 2. Anillin is expressed in cytoplasmic and nuclear fractions of the RCC cell line 786-O. Cytosolic (lane 1) and nuclear (lane 2) fractions were isolated and 20 μg of protein was loaded onto the SDS-PAGE gel. Western blotting with anillin antibody staining (upper blot) mainly shows expression in the nuclear fraction, but some expression is present in the cytosolic fraction. The size of anillin protein was identical in both fractions, ~120 kDa (upper blot and data not shown). Lamin A/C expression (lower blot) is present only in the nuclear fraction, excluding contamination of the cyto- solic fraction with nuclear content. Molecular weight markers (in kDa) are shown in the right margin. Representative blots from repeated experiments are shown.

Article Snippet: After boiling the samples in reducing SDS sample buffer for 5 min, 10 μg of protein was loaded per lane and the samples were electrophoresed on 10% polyacrylamide SDS gel and transferred to a PVDF membrane for anillin (IHC-00298, Bethyl Laboratories) or lamin A/C (NCL-LAM-A/C, Leica Biosystems Newcastle Ltd.) detection.

Techniques: Isolation, SDS Page, Western Blot, Staining, Expressing, Molecular Weight

Figure 3. Association between anillin cytoplasmic immunopositivity in RCCs and RCC-specific survival. The number of patients at risk with 0, 5 and 10 year follow-up is shown. The RCC-specific survival was signi- ficantly better among patients with cytoplasmic anillin immunopositive RCC (p=0.03).

Journal: Oncology Reports

Article Title: Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma

doi: 10.3892/or_00001051

Figure Lengend Snippet: Figure 3. Association between anillin cytoplasmic immunopositivity in RCCs and RCC-specific survival. The number of patients at risk with 0, 5 and 10 year follow-up is shown. The RCC-specific survival was signi- ficantly better among patients with cytoplasmic anillin immunopositive RCC (p=0.03).

Article Snippet: After boiling the samples in reducing SDS sample buffer for 5 min, 10 μg of protein was loaded per lane and the samples were electrophoresed on 10% polyacrylamide SDS gel and transferred to a PVDF membrane for anillin (IHC-00298, Bethyl Laboratories) or lamin A/C (NCL-LAM-A/C, Leica Biosystems Newcastle Ltd.) detection.

Techniques: